| 產(chǎn)品編號 | bs-0791R |
| 英文名稱 | CGRP-1/2 Rabbit pAb |
| 中文名稱 | 降鈣素基因相關肽1/2抗體 |
| 別 名 | CGRP-I+CGRP-II; CGRP-1/2; Procalcitonin; beta type CGRP; CALC2; CALCB; Calcitonin 1; Calcitonin 2; Calcitonin gene related peptide; Calcitonin gene related peptide II; Calcitonin related polypeptide beta; CGRP; CGRP II; CGRP2; CT; Calcitonin/calcitonin related polypeptide alpha; Katacalcin; MGC126648; CALCA_HUMAN; CALCB_HUMAN; Calcitonin gene-related peptide 2; beta-type CGRP; beta-CGRP; Calcitonin gene-related peptide II; CGRP-II; CGRP-I. |
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Specific References (13) | bs-0791R has been referenced in 13 publications.
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| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Cow,Chicken) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 3.8/14 kDa |
| 檢測分子量 | 4/14-17 |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calcitonin gene-related peptide 1: 65-130/130 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009] Function: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Subcellular Location: Secreted. Similarity: Belongs to the calcitonin family. SWISS: P01258 Gene ID: 796 Database links: Entrez Gene: 796 Human Entrez Gene: 797 Human Entrez Gene: 116903 Mouse Entrez Gene: 12310 Mouse Omim: 114130 Human Omim: 114160 Human SwissProt: P06881 Human SwissProt: P10092 Human SwissProt: P70160 Mouse SwissProt: Q99JA0 Mouse Unigene: 37058 Human Unigene: 534305 Human Unigene: 273838 Mouse Unigene: 4361 Mouse Unigene: 88080 Rat Unigene: 90085 Rat CGRP活性多肽，有37個氨基酸組成，具有多種生理功能，其藥理作用已受到廣泛重視. CGRP （又稱CGRP-1/2或CGRPa/β）廣泛分布于神經(jīng)系統(tǒng)的一種神經(jīng)肽，在外周及脊髓參與傷害性信息的傳遞及痛敏化的形成，并與P物質(zhì)、興奮性氨基酸、辣椒素1型受體、趨化因子、激活素、大麻素受體、阿片受體、神經(jīng)生長因子、血管活性腸肽、5-羥色胺、糖皮質(zhì)激素等生物活性物質(zhì)或受體在痛覺調(diào)制過程中存在相互影響。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CGRP) Polyclonal Antibody, Unconjugated (bs-0791R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CGRP/CGRP2 Polyclonal Antibody, Unconjugated(bs-0791R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
