| 產(chǎn)品編號 | bs-1309R |
| 英文名稱 | Syndecan-1 Rabbit pAb |
| 中文名稱 | 多配體蛋白聚糖1（CD138）抗體 |
| 別 名 | SDC1; SDC; SYND1; Syndecan I; SyndecanI; syndecan; Sdc1; Syndecan1; AA408134; AA409076; Sstn; syn-1; CD138; CD 138; CD-138; CD138 antigen; Heparan sulfate proteoglycan fibroblast growth factor receptor; SDC 1; Syndecan1 precursor; SDC1_HUMAN. |
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Specific References (9) | bs-1309R has been referenced in 9 publications.
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| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 細胞膜受體 細胞粘附分子 細胞表面分子 糖蛋白 上皮細胞 內(nèi)皮細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat,Cow (predicted: Rabbit,Pig,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | 60-70 |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD138: 201-310/310 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008] Function: Cell surface proteoglycan that bears both heparin sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Subunit: Interacts with CDCP1. Subcellular Location: Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form. Post-translational modifications: Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. Similarity: Belongs to the syndecan proteoglycan family. SWISS: P18827 Gene ID: 6382 Database links: Entrez Gene: 6382 Human Entrez Gene: 20969 Mouse Omim: 186355 Human SwissProt: P18827 Human SwissProt: P18828 Mouse Unigene: 224607 Human Unigene: 665958 Human Unigene: 2580 Mouse Unigene: 11176 Rat Syndecan1(CD138)屬于Syndecans基因家族，該家族是一類細胞表面跨膜蛋白多糖 ,是膜表面粘附受體 ,參與細胞-細胞外基質(zhì)之間的粘附。 CD138表達于上皮細胞、胚胎間質(zhì)細胞、血管平滑肌細胞、內(nèi)皮細胞和神經(jīng)細胞基底膜。Syndecan-1與惡性腫瘤的發(fā)生發(fā)展、浸潤及轉(zhuǎn)移有關(guān)，著色于胞漿和胞膜. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Human U251 cell lysates
Lane 4: Human A431 cell lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human HeLa cell lysates
Primary: Anti-Syndecan-1 (bs-1309R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 74 kDa
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
