| 產(chǎn)品編號(hào) | bs-0923R |
| 英文名稱 | PDX1 Rabbit pAb |
| 中文名稱 | 胰島素促進(jìn)因子/胰十二指腸同源異型盒蛋白抗體 |
| 別 名 | Glucose sensitive factor; Glucose-sensitive factor; GSF; IDX 1; IDX-1; IDX1; Insulin promoter factor 1; insulin promoter factor 1 homeodomain transcription factor; insulin upstream factor 1; IPF 1; IPF-1; IPF1; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF 1; IUF-1; IUF1; MODY4; Pancreas/duodenum homeobox 1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox P; PDX 1; PDX-1; PDX1_HUMAN; Somatostatin transactivating factor 1; Somatostatin-transactivating factor 1; STF 1; STF-1; STF1. |
 |
Specific References (6) | bs-0923R has been referenced in 6 publications.
[IF=8.025] Bing Yang. et al. Polysaccharide from Hovenia dulcis (Guaizao) improves pancreatic injury and regulates liver glycometabolism to alleviate STZ-induced type 1 diabetes mellitus in rats. INT J BIOL MACROMOL. 2022 Jun;: WB ; Rat.
[IF=5.29] Zheng, Liming, et al. "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." Scientific Reports 6 (2016): 37414. IF(IHC-P) ; Goat.
[IF=4.546] Chi-Hao Tsaiet al. The Powdered Root of Eurycoma longifolia Jack Improves Beta-Cell Number and Pancreatic Islet Performance through PDX1 Induction and Shows Antihyperglycemic Activity in db/db Mice. Nutrients
. 2020 Jul 16;12(7):2111. IHC ; mouse.
[IF=2.08] Jian, Ruo-Lei, et al. "Generation of insulin-producing cells from C3H10T1/2 mesenchymal progenitor cells." Gene (2015). WB ; Mouse.
[IF=1.64] Hefei, Wang, et al. "Morphological Characteristics and Identification of Islet‐like Cells Derived From Rat Adipose‐derived Stem Cells Cocultured with Pancreas Adult Stem Cells." Cell Biology International (2014). Rat.
[IF=0] Herawati L et al. Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice. Diabetes mellitus, 2019,21(6):497-505. IHC ; Mouse.
|
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 生長(zhǎng)因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 內(nèi)分泌病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDX1: 188-284/284 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Aug 2017] Function: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Subunit: Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Subcellular Location: Nucleus. Tissue Specificity: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Post-translational modifications: Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. DISEASE: Defects in PDX1 are a cause of pancreatic agenesis (PAC)[MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant. Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Similarity: Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. Contains 1 homeobox DNA-binding domain. SWISS: P52945 Gene ID: 3651 Database links: Entrez Gene: 3651 Human Entrez Gene: 18609 Mouse Omim: 600733 Human SwissProt: P52945 Human SwissProt: P52946 Mouse Unigene: 32938 Human Unigene: 389714 Mouse Unigene: 54603 Rat PDX1是一種同源框轉(zhuǎn)錄因子-即胰十二指腸同源異型盒基因，又稱IPF-1(胰島素促進(jìn)因子)、IDX-1、IUF-1。 近年來(lái)，科學(xué)家們對(duì)PDX1從不同的角度進(jìn)行了卓有成效的研究。有學(xué)者認(rèn)為；PDX1是胰腺發(fā)育及胰島素基因轉(zhuǎn)錄表達(dá)的關(guān)鍵性轉(zhuǎn)錄因子，即決定于胰腺前體細(xì)胞向B、A、D細(xì)胞的分化。 還有學(xué)者認(rèn)為：PDX1對(duì)于腸內(nèi)胚層背胰芽和腹胰芽的生長(zhǎng)、分化起重要作用，早期胰腺表達(dá)的PDX-1對(duì)胰腺上皮的形成和分化是必需的。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PDX-1 Polyclonal Antibody, Unconjugated(bs-0923R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
