| 產(chǎn)品編號 | bs-1827R |
| 英文名稱 | IL-11 Rabbit pAb |
| 中文名稱 | 白介素11抗體 |
| 別 名 | Interleukin-11; Adipogenesis inhibitory factor; AGIF; IL 11; IL11; Interleukin 11; Oprelvekin; IL11_HUMAN. |
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Specific References (2) | bs-1827R has been referenced in 2 publications.
[IF=5.555] Pengsen Wu. et al. IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy. Front Endocrinol. 2022; 13: 846106 IHC ; Human.
[IF=2.376] Lifei Li. et al. GnRH agonist treatment regulates IL-6 and IL-11 expression in endometrial stromal cells for patients with HRT regiment in frozen embryo transfer cycles. Reprod Biol. 2022 Jun;22:100608 WB ; Human.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 血管內(nèi)皮細(xì)胞 淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-11: 22-120/199 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production.
Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy. Function: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Subcellular Location: Secreted. Similarity: Belongs to the IL-6 superfamily. SWISS: P20809 Gene ID: 3589 Database links: Entrez Gene: 3589 Human Omim: 147681 Human SwissProt: P20809 Human Unigene: 467304 Human 白介素11，是一種多向性細(xì)胞因子。很多細(xì)胞都能合成IL11，如：內(nèi)皮細(xì)胞、纖維母細(xì)胞、軟骨細(xì)胞、滑膜細(xì)胞、成骨細(xì)胞、上皮細(xì)胞、神經(jīng)元、胸腺等。正常的血清中，IL11表達(dá)很少或處在檢測水平之下。對造血系統(tǒng)的功能包括：干細(xì)胞、巨核細(xì)胞、骨髓細(xì)胞、紅細(xì)胞和B淋巴細(xì)胞的增生和發(fā)育。對造血系統(tǒng)之外也具有多相性的作用，有些是刺激和促進(jìn)增生，有些是抑制作用。人、大鼠、小鼠的IL-11的前體有199個(gè)氨基酸。成熟形態(tài)為178個(gè)氨基酸。 |
| 產(chǎn)品圖片 |
Sample: Testis (Mouse) Lysate at 30 ug
Primary: Anti- IL-11 (bs-1827R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-IL-11 (bs-1827R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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