| 產(chǎn)品編號 | bs-2309R |
| 英文名稱 | PPV VP2 Rabbit pAb |
| 中文名稱 | 豬細小病毒VP2抗體 |
| 別 名 | PPV-VP2; Porcine parvovirus VP2; PPV Isoform VP2(P18546-2); Capsid protein VP1; CAPSD_PAVPN. |
| 研究領(lǐng)域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Porcine parvovirus) |
| 產(chǎn)品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Porcine Parvovirus VP2 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Porcine Parvovirus Infection (PPV) is the most common and important cause of infectious infertility. Porcine parvovirus is a fairly tough virus that multiplies normally in the intestine of the pig without causing clinical signs. It is world-wide in its distribution. If you test for it in your pig herd it is almost certain it will be present unless your herd is less than 100 sows when it might have died out. It is therefore an infection you have to live with and manage. Whereas most viruses do not survive outside the host for any great period of time PPV is unusual in that it can persist outside the pig for many months and it is resistant to most disinfectants. This perhaps explains why it is so widespread and so difficult to remove from the pig environment. Function: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus (By similarity). 豬細小病毒(Porcine parvovirus,PPV)是引起豬繁殖障礙的重要病毒性傳染病。病毒經(jīng)口、鼻等黏膜感染是其主要感染途徑。懷孕母豬感染后可通過胎盤感染引起母豬流產(chǎn)、死胎、木乃伊胎及新生仔豬死亡。因該病流行面廣,危害嚴重,給養(yǎng)豬業(yè)帶來重大經(jīng)濟損失。PPV基因組編碼3種結(jié)構(gòu)蛋白,分別是VP1、VP2、VP3,其中VP2是構(gòu)成病毒粒子的主要衣殼蛋白,約占病毒衣殼蛋白總量的80%,VP2蛋白攜帶主要的抗原決定簇,可誘導機體產(chǎn)生中和抗體,VP2對病毒感染、發(fā)揮其致病性方面亦起關(guān)鍵作用。因此,PPV-VP2蛋白在PPV診斷和免疫防治等有著很重要的意義。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
