| 產(chǎn)品編號 | bs-2399R |
| 英文名稱 | CYP7A1 Rabbit pAb |
| 中文名稱 | 細胞色素P450 7A1抗體/膽固醇7a羥化酶抗體 |
| 別 名 | Cholesterol 7 alpha hydroxylase; Cholesterol 7 alpha monooxygenase; cholesterol 7 alpha-monooxygenase; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CP7A; CYP 7; CYP7; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; Cytochrome P450, subfamily VIIA(cholesterol 7 alpha monooxygenase); CP7A1_HUMAN. |
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Specific References (19) | bs-2399R has been referenced in 19 publications.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,GuineaPig (predicted: Mouse,Rat,Rabbit,Pig) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP7A1: 351-400/504 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]. Function: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Detected in liver. Similarity: Belongs to the cytochrome P450 family. SWISS: P22680 Gene ID: 1581 Database links: Entrez Gene: 1581 Human Entrez Gene: 13122 Mouse Omim: 118455 Human SwissProt: P22680 Human SwissProt: Q64505 Mouse Unigene: 1644 Human Unigene: 57029 Mouse Unigene: 10737 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (bs-2399R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (bs-2399R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
