| 產(chǎn)品編號 | bs-4589R |
| 英文名稱 | High density lipoprotein Rabbit pAb |
| 中文名稱 | 高密度脂蛋白抗體 |
| 別 名 | HDL. |
| 研究領(lǐng)域 | 心血管 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | human high density lipoprotein purified from plasma |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
High-density lipoproteins (HDL) are one of the five major groups of lipoproteins. Lipoproteins are complex particles composed of multiple proteins which transport all fat molecules (lipids) around the body within the water outside cells. They are typically composed of 80-100 proteins per particle (organized by one, two or three ApoA; more as the particles enlarge picking up and carrying more fat molecules) and transporting up to hundreds of fat molecules per particle. Unlike the larger lipoprotein particles which deliver fat molecules to cells, HDL particles remove fat molecules from cells which need to export fat molecules. The fats carried include cholesterol, phospholipids, and triglycerides; amounts of each are quite variable. Gene ID: HDL 高密度脂蛋白（HDL）為血清蛋白之一，又稱載脂蛋白A。高密度脂蛋白在肝、腸和血液中合成，擔負著將內(nèi)源性膽固醇(以膽固醇酯為主)從組織往肝臟的逆向轉(zhuǎn)運。血漿高密度脂蛋白含量的高低與患心血管病的風險呈負相關(guān)。比較富含磷脂質(zhì)，在血清中的含量約為300mg／dl。其蛋白質(zhì)部分， A－Ⅰ約為75％， A－Ⅱ約為20％。由于可輸出膽固醇促進膽固醇的代謝，所以現(xiàn)在作為動脈硬化預防因子而受到重視。高密度脂蛋白運載周圍組織中的膽固醇，再轉(zhuǎn)化為膽汁酸或直接通過膽汁從腸道排出，高密度脂蛋白膽固醇含量與動脈管腔狹窄程度呈顯著的負相關(guān)。所以高密度脂蛋白是一種抗動脈粥樣硬化的血漿脂蛋白，是冠心病的保護因子，俗稱“血管清道夫”。 HDL在生理上起著將肝外組織的膽固醇運送到肝臟的運載工具的作用，因而可以防止游離膽固醇在肝外組織細胞上的沉積，高密度脂蛋白膽固醇對冠心病的臨床診斷是一個重要的參考指標。它的降低是臨床冠心病的危險因子之一，并可能促進動脈粥樣硬化的發(fā)展。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Serum
Primary: Anti-High density lipoprotein (bs-4589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28kDa
Observed band size: 28kDa
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (High density lipoprotein) Polyclonal Antibody, Unconjugated (bs-4589R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
