| 產(chǎn)品編號(hào) | bs-6196R |
| 英文名稱 | Transglutaminase 2 Rabbit pAb |
| 中文名稱 | 谷氨酰胺轉(zhuǎn)胺酶2抗體 |
| 別 名 | ALPHA SUBUNIT; C polypeptide; EC 2.3.2.13; G alpha h; G protein alpha subunit Gh class; G[a]h; Gh CLASS G ALPHA h; GNAH; GNAH G PROTEIN; Guanine nucleotide binding protein H polypeptide; H POLYPEPTIDE; Protein glutamine gamma glutamyltransferase 2; Protein glutamine gamma glutamyltransferase; Protein-glutamine gamma-glutamyltransferase 2; TG 2; TG(C); TG2; TGase C; TGase C; TGase H; TGase-2; TgaseII; TGC; TGC GUANINE NUCLEOTIDE BINDING PROTEIN; TGM 2; TGM2; TGM2; TGM2_HUMAN; Tissue transglutaminase; Tissue type transglutaminase; Transglutaminase 2; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase-2; tTG; tTGas. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 77 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGM2.: 551-650/687 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. Function: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Subunit: Monomer. Similarity: Belongs to the transglutaminase superfamily. Transglutaminase family. SWISS: P21980 Gene ID: 7052 Database links: Entrez Gene: 7052 Human Entrez Gene: 21817 Mouse Omim: 190196 Human SwissProt: P21980 Human SwissProt: P21981 Mouse Unigene: 517033 Human Unigene: 330731 Mouse 谷氨酰胺轉(zhuǎn)氨酶，簡(jiǎn)稱TG。微生物谷氨酰胺轉(zhuǎn)胺酶在自然界中廣泛存在于動(dòng)物、植物和微生物中，它是一種?；D(zhuǎn)移酶一種催化蛋白質(zhì)間（或多肽）發(fā)生共價(jià)交聯(lián)的酶，這種交聯(lián)對(duì)蛋白質(zhì)的性質(zhì)，凝聚能力，熱穩(wěn)定，含水能力有著顯著的影響。目前也是食品行業(yè)中很常用的食品添加劑。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGM2 Polyclonal Antibody, Unconjugated(bs-6196R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGM2 Polyclonal Antibody, Unconjugated(bs-6196R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGM2 Polyclonal Antibody, Unconjugated(bs-6196R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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