| 產(chǎn)品編號(hào) | bs-12401R |
| 英文名稱(chēng) | NLE1 Rabbit pAb |
| 中文名稱(chēng) | NLE1蛋白抗體 |
| 別 名 | FLJ10458; NLE 1; Nle; Notchless gene homolog; Notchless homolog 1(Drosophila); Notchless homolog 1; Notchless protein homolog 1; NLE1_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NLE1: 351-450/485 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway. In plants, NLE1 is crucial for normal cellular growth and development. Under-expression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing. Function: NLE1 (Notchless protein homolog 1) contains 8 WD repeats and may be involved in the Notch signaling pathway. Subcellular Location: Nuclear, nucleolus. Similarity: Belongs to the NLE1/RSA4 family. Contains 8 WD repeats. SWISS: Q9NVX2 Gene ID: 54475 Database links: Entrez Gene: 54475 Human Entrez Gene: 217011 Mouse SwissProt: Q9NVX2 Human SwissProt: Q8VEJ4 Mouse Unigene: 85570 Human Unigene: 29176 Mouse Unigene: 19087 Rat |
| 產(chǎn)品圖片 |
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-NLE1 (bs-12401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-NLE1 (bs-12401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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