| 產(chǎn)品編號 | bs-13536R |
| 英文名稱 | GPR43 Rabbit pAb |
| 中文名稱 | G蛋白偶聯(lián)受體43抗體 |
| 別 名 | GPCR GPR43; FFA2R; Ffar2; FFAR2_HUMAN; free fatty acid activated receptor 2; Free fatty acid receptor 2; G protein coupled receptor 43; G protein-coupled receptor 43; G-protein coupled receptor 43; GPR 43; GPCR 43. |
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Specific References (16) | bs-13536R has been referenced in 16 publications.
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| 研究領域 | 細胞生物 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR43: 41-140/330 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
G protein-coupled receptors provide attractive targets for drug therapy due to the sheer size and diversity of ligands within this receptor family. G protein-coupled receptor (GPR) GPR41 and GPR43 are related members of a homologous family of orphan G protein-coupled receptors that are tandemly encoded at a single chromosomal locus in both humans and mice. GPR43 functions as a ligand for short chain fatty acids (SCFAs), notably acetate and propionate. Bacteria in the gut produce high concentrations of SCFAs, which are subsequently released in the bloodstream, where they exert cellular effects on blood leukocytes, including calcium release, ERK1/2 activation, and inhibition of cAMP accumulation. These effects indicate a role for GPR43 in the recruitment of leukocytes, particularly polymorphonuclear cells, to sites of bacterial infection. Function: Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Subcellular Location: Cell membrane. Tissue Specificity: Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O15552 Gene ID: 2867 Database links: Entrez Gene: 2867 Human Omim: 603823 Human SwissProt: O15552 Human Unigene: 248056 Human |
| 產(chǎn)品圖片 |
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR43 Polyclonal Antibody, Unconjugated(bs-13536R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GPR43) polyclonal Antibody, Unconjugated (bs-13536R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
