| 產品編號 | bs-14324R |
| 英文名稱 | DHX8 Rabbit pAb |
| 中文名稱 | DHX8蛋白抗體 |
| 別 名 | ATP dependent RNA helicase DHX8; ATP-dependent RNA helicase dhx8; DDX 8; DDX8; DEAD/H(Asp Glu Ala Asp/His) box polypeptide 8(RNA helicase); DEAD/H(Asp Glu Ala Asp/His) box polypeptide 8; DEAD/H box polypeptide 8; DEAH(Asp Glu Ala His) box polypeptide 8; DEAH box polypeptide 8; DEAH box protein 8; DHX 8; dhx8; DHX8_HUMAN; HRH 1; HRH1; PRP 22; PRP22; PRPF 22; PRPF22; RNA helicase HRH 1; RNA helicase HRH1. |
| 研究領域 | 細胞生物 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 139 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHX8: 1101-1220/1220 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. [provided by RefSeq, Jul 2008] Function: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Subunit: Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Subcellular Location: Nucleus. Similarity: Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 S1 motif domain. SWISS: Q14562 Gene ID: 1659 Database links: Entrez Gene: 1659 Human Entrez Gene: 217207 Mouse Omim: 600396 Human SwissProt: Q14562 Human SwissProt: A2A4P0 Mouse Unigene: 463105 Human Unigene: 28186 Mouse |
| 產品圖片 |
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DHX8 Polyclonal Antibody, Unconjugated(bs-14324R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
