殿上欢,玄幻小说完本

產(chǎn)品編號	bs-10900R
英文名稱	GAPDH Rabbit pAb, Loading Control
中文名稱	3-磷酸甘油醛脫氫酶（內(nèi)參）抗體
別名	38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.
	Specific References (53) \| bs-10900R has been referenced in 53 publications. [IF=8.101] Leyi Yao. et al. Plumbagin is a novel GPX4 protein degrader that induces apoptosis in hepatocellular carcinoma cells. FREE RADICAL BIO MED. 2023 Jul;203:1 WB ; Human. PubMed:37011699 [IF=7.917] Huang Shu. et al. Targeting nano-regulator based on metal–organic frameworks for enhanced immunotherapy of bone metastatic prostate cancer. CANCER NANOTECHNOL. 2023 Dec;14(1):1-15 WB ; Mouse,Human. PubMed:10.1186/s12645-023-00200-y [IF=7.74] Qifeng Deng . et al. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin?ferroportin axis. Gut Microbes. 2021;13(1):1-18 WB ; Mouse. PubMed:33475464 [IF=7.666] Valeria Lucchino. et al. Insights into the Genetic Profile of Two Siblings Affected by Unverricht-Lundborg Disease Using Patient-Derived hiPSCs. CELLS-BASEL. 2022 Jan;11(21):3491 WB ; Human. PubMed:36359887 [IF=6.832] Tao Cai. et al. Long noncoding RNA BBOX1-AS1 promotes the progression of gastric cancer by regulating the miR-361-3p/Mucin 13 signaling axis. BIOENGINEERED. 2022 Jun 05 WB ; Human. PubMed:10.1080/21655979.2022.2072629 [IF=6.706] Zhen-Xing Wang. et al. The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells, as Affected by the Covalent Se Conjugation. NUTRIENTS. 2022 Jan;14(19):3950 WB ; Rat. PubMed:36235602 [IF=6.34] Zhou et al. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression. (2017) Redox.Biol. 11:390-402 WB ; Rat, Human. PubMed:28063381 [IF=6.17] Yinyun Ni. et al. miR-15a-5p inhibits metastasis and lipid metabolism by suppressing histone acetylation in lung cancer. Free Radical Bio Med. 2020 Dec;161:150 WB ; Human. PubMed:33059020 [IF=5.81] Zhou Z. et al. Vanillin Derivatives Reverse Fusobacterium nucleatum-Induced Proliferation and Migration of Colorectal Cancer Through E-Cadherin/β-Catenin Pathway.. Front Pharmacol. 2022 Mar;13:841918-841918 WB ; Human. PubMed:35308221 [IF=5.714] Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:109340 WB ; Mouse. PubMed:36330910 [IF=5.195] Yingjie Duan. et al. VX-765 ameliorates CKD VSMC calcification by regulating STAT3 activation. EUR J PHARMACOL. 2023 Apr;945:175610 WB ; Rat. PubMed:36858340 [IF=5.008] Liang et al. Itraconazole exerts its anti-melanoma effect by suppressing Hedgehog, Wnt, and PI3K/mTOR signaling pathways. (2017) Oncotarge. 8:28510-28525 WB ; Human. PubMed:28212537 [IF=5.008] Mu et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer. (2017) Oncotarge. 8:39443-39459 WB ; Human. PubMed:28467796 [IF=4.784] Liu XQ et al. Sodium tanshinone IIA sulfonate protects against Aβ1–42-induced cellular toxicity by modulating Aβ-degrading enzymes in HT22 cells. Int J Biol Macromol. 2020 Feb 6;151:47-55. WB ; Mouse. PubMed:32035959 [IF=4.652] Lin et al. MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells. (2017) Cell.Physiol.Bioche. 41:124-136 WB ; Rat. PubMed:28214900 [IF=4.652] Zong et al. The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro. (2016) Cell.Physiol.Bioche. 38:1100-10 WB ; Mouse. PubMed:26938714 [IF=4.631] Shifei Li. et al. miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus. J INFLAMM RES. 2022 Dec;15:6607-6616 WB ; Human. PubMed:36510495 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.486] Meiling Zhou. et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. 2021 Mar 01 WB ; Rat. PubMed:33645011 [IF=4.302] Jia et al. Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α. (2018) J.Cell.Mol.Med. 22:4507-4521 WB ; Mouse. PubMed:29993193 [IF=4.268] Wu H et al. A UPLC-Q-TOF/MS-based plasma metabolomics approach reveals the mechanism of Compound Kushen Injection-based intervention against non-small cell lung cancer in Lewis tumor-bearing mice. Phytomedicine . 2020 Jun 2;76:153259. WB ; Mouse. PubMed:32534358 [IF=4.175] Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15 WB ; PubMed:33407505 [IF=4.175] Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12 WB ; Human. PubMed:33292220 [IF=4.123] Sun L et al. Salmonella Effector SpvB Disrupts Intestinal Epithelial Barrier Integrity for Bacterial TranslocationFront Cell Infect Microbiol.2020 Dec 17;10:606541. WB ; Human. PubMed:33392110 [IF=3.647] Lin X et al. Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids. Life Sci . 2020 Aug 15;255:117859. WB ; Human. PubMed:32474020 [IF=3.641] Baozhen Qu. et al. Preserved antibacterial activity of ribosomal protein S15 during evolution. Mol Immunol. 2020 Nov;127:57 WB ; Shrimp, Amphioxus, Zebrafish and Mouse. PubMed:32927165 [IF=3.598] Chang L et al. Gypenoside A protects ischemia/reperfusion injuries by suppressing miR‐143‐3p level via the activation of AMPK/Foxo1 pathway. Biofactors. 2019 Dec 30. WB ; Rat. PubMed:31889343 [IF=3.558] Jie Cui. et al. A zinc finger family protein, ZNF263, promotes hepatocellular carcinoma resistance to apoptosis via activation of ER stress-dependent autophagy. Transl Oncol. 2020 Dec;13:100851 WB ; Human. PubMed:32898766 [IF=3.481] Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25 WB ; Human. PubMed:34032284 [IF=3.448] Wang T et al. Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Life Sci. 2020 Feb 13;247:117421. WB ; Rat. PubMed:32061865
產(chǎn)品類型	內(nèi)參抗體
研究領(lǐng)域	腫瘤細(xì)胞生物免疫學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝
抗體來源	Rabbit
克隆類型	Polyclonal
克隆號
交叉反應(yīng)	Human,Mouse,Rat,Hamster
產(chǎn)品應(yīng)用	WB=1:5000-50000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	38 kDa
檢測分子量
細(xì)胞定位	細(xì)胞核細(xì)胞漿細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	Recombinant human GAPDH full length protein
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subunit: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS. Subcellular Location: Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. Post-translational modifications: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated (Probable). Sulfhydration at Cys-152 increases catalytic activity. Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. SWISS: P04406 Gene ID: 2597 Database links: Entrez Gene: 374193 Chicken Entrez Gene: 2597 Human Entrez Gene: 100042025 Mouse Entrez Gene: 14433 Mouse Entrez Gene: 24383 Rat Entrez Gene: 685186 Rat Entrez Gene: 317743 Zebrafish Omim: 138400 Human SwissProt: P00356 Chicken SwissProt: P04406 Human SwissProt: P16858 Mouse SwissProt: P04797 Rat SwissProt: Q5XJ10 Zebrafish GAPDH蛋白幾乎在所有組織中都高水平表達(dá)，廣泛用作Western blot蛋白質(zhì)標(biāo)準(zhǔn)化的內(nèi)參,是很好的內(nèi)參抗體。 GAPDH 作為管家基因在同種細(xì)胞或者組織中的蛋白質(zhì)表達(dá)量一般是恒定的。在實驗中，可能存在總蛋白濃度測定不準(zhǔn)確；或者蛋白質(zhì)樣品在電泳前上樣時產(chǎn)生的樣品間的操作誤差；這些誤差需要通過測定每個樣品中實際轉(zhuǎn)到膜上的GAPDH的含量來進行校正，所以一般的western實驗都需要進行內(nèi)參設(shè)置。具體校正的方法就是將每個樣品測得的目的蛋白含量與本樣品的GAPDH含量相除，得到每個樣品目的蛋白的相對含量。然后才進行樣品與樣品之間的比較。甘油醛-3-磷酸脫氫酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）過程中的關(guān)鍵酶。除了在胞質(zhì)中作為糖酵解的酶以外，有證據(jù)表明哺乳動物細(xì)胞中的GAPDH參與了多種胞內(nèi)生化過程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸轉(zhuǎn)移酶（phosphotransferase）激活、核內(nèi)RNA出核、DNA復(fù)制與DNA修復(fù)。一些生理因素，諸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定細(xì)胞中的表達(dá)。GAPDH存在于幾乎所有的組織中，以高水平持續(xù)表達(dá)。 GAPDH（甘油醛-3-磷酸脫氫酶）是參與糖酵解的一種關(guān)鍵酶，由4個30－40kDa的亞基組成.
產(chǎn)品圖片	Sample: Lane 1,4,7: Mouse Cerebellum tissue lysates Lane 2,5,8: Rat Cerebellum tissue lysates Lane 3,6,9: Human SH-SY5Y cell lysates Primary: Anti-GAPDH (bs-10900R) at 1/10000~1/100000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 36 kDa Sample: Lane 1: Cerebellum (Mouse) Lysate Lane 2: Raw264.7 (Mouse) Cell Lysate Lane 3: NIH/3T3 (Mouse) Cell Lysate Lane 4: Cerebrum (Rat) Lysate Lane 5: SH-SY5Y (Human) Cell Lysate Lane 6: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH (bs-10900R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價測定程序

	AbBy Fluor 350 標(biāo)記
	AbBy Fluor 488 標(biāo)記
	AbBy Fluor 555 標(biāo)記
	AbBy Fluor 594 標(biāo)記
	AbBy Fluor 647 標(biāo)記
	AP 標(biāo)記
	APC 標(biāo)記
	Biotin 標(biāo)記
	Cy3 標(biāo)記
	Cy5 標(biāo)記
	Cy5.5 標(biāo)記
	Cy7 標(biāo)記
	FITC 標(biāo)記
	Gold 標(biāo)記
	HRP 標(biāo)記
	PE 標(biāo)記
	PE-Cy3 標(biāo)記
	PE-CY5 標(biāo)記
	PE-CY5.5 標(biāo)記
	PE-CY7 標(biāo)記
	RBITC 標(biāo)記