| 產(chǎn)品編號(hào) | bs-23699R |
| 英文名稱 | TFPI-2 Rabbit pAb |
| 中文名稱 | 組織因子途徑抑制劑-2抗體 |
| 別 名 | tissue factor pathway inhibitor 2; TFPI-2; TFPI2; TFPI2_HUMAN; Tissue factor pathway inhibitor 2; Placental protein 5; PP5; TFPI 2; TFPI 2; TFPI2 PEN. |
| 研究領(lǐng)域 | 心血管 細(xì)胞生物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TFPI-2: 1-100/230 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TFPI2 may play a role in the regulation of plasmin-mediated matrix remodeling. It inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa, but it has no effect on thrombin. TFPI2 is specific to umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. This inhibitor contains three inhibitory domains. TFPI2 contains 3 BPTI/Kunitz inhibitor domains. Function: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. Subcellular Location: Secreted (constituent of proteinaceous extracellular matrix) Tissue Specificity: Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. Similarity: Contains 3 BPTI/Kunitz inhibitor domains. SWISS: P48307 Gene ID: 7980 Database links: Entrez Gene: 7980 Human Omim: 600033 Human SwissProt: P48307 Human Unigene: 438231 Human 組織因子途徑抑制因子(tissue factor pathway in hibitor, TFPI)是組織因子凝血途徑的主要抑制因子， 組織因子途徑抑制因子在諸如動(dòng)脈粥樣硬化，血管再狹窄，彌漫性血管內(nèi)凝血DIC等方面，有一定的研究?jī)r(jià)值。 TFPI-2也稱為胎盤(pán)蛋白-5(placenta protein 5，PP5)或細(xì)胞基質(zhì)聯(lián)合絲氨酸蛋白酶抑制物(matrix associated serine protease inhibitor，MSPI)。 TFPI-2是相對(duì)分子質(zhì)量為:25KDa的絲氨酸蛋白酶抑制物, 由脈管系統(tǒng)細(xì)胞（內(nèi)皮細(xì)胞、平滑肌細(xì)胞、成纖維細(xì)胞）合成，并沉積在這些細(xì)胞的細(xì)胞外基質(zhì)里。TFPI-2為廣譜絲氨酸蛋白酶抑制劑，能有效地抑制基質(zhì)金屬蛋白酶（MMP）、纖溶酶、胰蛋白酶、糜蛋白酶、組織蛋白酶等多種蛋白水解酶的活性，在維持細(xì)胞外基質(zhì)（ECM）結(jié)構(gòu)完整、抑制腫瘤細(xì)胞浸潤(rùn)轉(zhuǎn)移方面起著重要作用。TFPI-2分布廣泛,在人肝臟、腎臟、心臟、骨骼肌等正常組織中有著高度表達(dá),而在腫瘤組織中也有一定程度的表達(dá)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFPI-2) Polyclonal Antibody, Unconjugated (bs-23699R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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