| 產(chǎn)品編號(hào) | bs-43004R |
| 英文名稱 | IGFBP1 Rabbit pAb |
| 中文名稱 | 胰島素樣生長因子結(jié)合蛋白1抗體 |
| 別 名 | Insulin-like Growth Factor Binding Protein 1; IGFBP-1; AFBP; alpha pregnancy associated endometrial globulin; Amniotic fluid binding protein; Binding protein 25; Binding protein 26; Binding protein 28; Growth hormone independent binding protein; hIGFBP 1; hIGFBP1; IBP 1; IBP1; IGF binding protein 1; IGF BP25; IGFBP 1; Placental protein 12; PP12. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human IGFBP1 protein: 26-259/259 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Insulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner. Post translational modification of IGFBPs, including phosphorylation and proteolysis, will modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP1 is expressed in liver, decidua, and kidneys and is the major IGF binding protein in human amnionic fluid. In hepatocytes, IGFBP1 production is regulated at the transcriptional level due to the effects of insulin and corticosteriods. IGFBP1 is the major determinant of the level of free IGF in serum. The expression of IGFBP1 is inhibited by insulin, IGFI, and IGFII and is stimulated by glucocorticoids, thyroid hormone, and epidermal growth factor (EGF), indicating an endocrine function. IGFBP1 shows inhibitory actions on cell proliferation and differentiation, presumably by interfering with the interactions between IGF and the IGF receptor (IGFR). Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. Subunit: Binds equally well IGF1 and IGF2. Subcellular Location: Secreted. Post-translational modifications: Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. SWISS: P08833 Gene ID: 3484 Database links: Entrez Gene: 3484 Human Entrez Gene: 16006 Mouse Omim: 146730 Human SwissProt: P08833 Human SwissProt: P47876 Mouse Unigene: 642938 Human Unigene: 21300 Mouse Unigene: 34026 Rat 生長因子和激素（ Growth Factor and Hormones） 胰島素樣生長因子結(jié)合蛋白1(IGFBP-2)是IGF/IGFBP家族中的一個(gè)重要成員,在多種疾病尤其是腫瘤中其表達(dá)明顯增高,可能通過調(diào)節(jié)胰島素樣生長因子(insulin-like growth factor,IGF)發(fā)揮作用，促進(jìn)腫瘤的發(fā)生發(fā)展。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human IGFBP1 Protein at 500ng
Lane 2: Human IGFBP1 Protein at 50ng
Primary: Rabbit Anti-IGFBP1 Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29kD
Observed band size: kD
Sample:
Lane 1: Recombinant human IGFBP1 protein, His (HEK293) (bs-43004P)
Primary: Anti-IGFBP1 (bs-43004R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30kDa
Observed band size: 30kDa
|
