| 產品編號 | bs-41322R |
| 英文名稱 | CH25H Rabbit pAb |
| 中文名稱 | 膽固醇25羥化酶抗體 |
| 別 名 | C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | 32 |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CH25H protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. Function: CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Subcellular Location: Endoplasmic reticulum membrane Post-translational modifications: N-glycosylated. Similarity: Belongs to the sterol desaturase family. SWISS: O95992 Gene ID: 9023 Database links: Entrez Gene: 9023 Human Entrez Gene: 12642 Mouse Omim: 604551 Human SwissProt: O95992 Human SwissProt: Q9Z0F5 Mouse |
| 產品圖片 |
Sample:
Lane 1: Recombinant human CH25H protein, His(bs-41322P)
Primary: Anti-CH25H (bs-41322R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 11 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (bs-41322R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
