| 產(chǎn)品編號 | bsm-60591R |
| 英文名稱 | PAX9 Recombinant Rabbit mAb |
| 中文名稱 | 配對盒基因9重組兔單抗 |
| 別 名 | Paired box 9; Paired box gene 9; Paired box homeotic gene 9; Paired box protein 9; Paired box protein Pax 9; Paired box protein Pax9; Paired domain gene 9; PAX 9; PAX9_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | G12B2 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. Mice lacking this gene exhibit impaired development of organs, musculature and the skeleton, including absent and abnormally developed teeth, and neonatal lethality. Mutations in the human gene are associated with selective tooth agenesis-3. [provided by RefSeq, Sep 2015] PAX9是一種在人類體內(nèi)由PAX9基因編碼的蛋白質(zhì)。該基因是轉(zhuǎn)錄因子PAX家族中的一員。在小鼠胚胎發(fā)生過程中，PAX9從胚胎期第8.5天開始表達，到胚胎期第9.5天時更加明顯；在這個階段，它的表達僅限于咽內(nèi)胚層。隨后，PAX9逐漸在軸向骨架中表達。PAX9是顱面、牙齒和肢體發(fā)育所必需的，也可能涉及到鱗狀上皮以及各種器官及骨骼的發(fā)育。在某些人群中，PAX9在智齒缺失中發(fā)揮了一定的作用。同時，它也是胸腺、甲狀旁腺、鰓下體、牙齒、顱骨和喉部的骨骼以及遠端肢體正常發(fā)育所必需的轉(zhuǎn)錄因子。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with PAX9 monoclonal antibody, unconjugated (bsm-60591R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PAX9) monoclonal Antibody, unconjugated (bsm-60591R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
