| 產(chǎn)品編號(hào) | bsm-56206R |
| 英文名稱 | Myogenin Recombinant Rabbit mAb |
| 中文名稱 | 肌細(xì)胞生成素重組兔單抗 |
| 別 名 | MYOG_HUMAN; MYOG_MOUSE; MYOG; BHLHC3; MYF4; Class C basic helix-loop-helix protein 3(bHLHc3); Myogenic factor 4(Myf-4); BHLHC3; MYF4; MYF 4; |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 2B16 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Myogenin: 1-48 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts. Subunit: Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminus region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex. Subcellular Location: Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos. Post-translational modifications: Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P15173 Gene ID: 4656 Database links: Entrez Gene: 4656 Human Entrez Gene: 17928 Mouse SwissProt: P15173 Human SwissProt: P12979 Mouse MYOG是肌肉中運(yùn)載氧的蛋白質(zhì)，由153個(gè)氨基酸殘基組成，一種小分子蛋白質(zhì)，含有血紅素，和血紅蛋白同源，與氧的結(jié)合能力介于血紅蛋白和細(xì)胞色素氧化酶之間，可幫助肌細(xì)胞將氧轉(zhuǎn)運(yùn)到線粒體。 肌細(xì)胞生成素在成肌分化過(guò)程中起著決定性的作用。在人體骨骼肌的胚胎發(fā)育過(guò)程中 ,肌細(xì)胞生成素伴隨成肌過(guò)程而呈高水平表達(dá)。MYOG主要由腎臟代謝并排泄，當(dāng)心肌或橫紋肌有損傷時(shí)，肌紅蛋白便釋放入血中，血清中的肌紅蛋白即可明顯升高。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myogenin Monoclonal Antibody, Unconjugated(bsm-56206R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myogenin Monoclonal Antibody, Unconjugated(bsm-56206R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Rhabdomyosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Myogenin Monoclonal Antibody, Unconjugated(bsm-56206R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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