| 產(chǎn)品編號 | bsm-60916M |
| 英文名稱 | S100A4 Mouse mAb |
| 中文名稱 | FSP-1單克隆抗體 |
| 別 名 | FSP1; FSP-1; FSP 1; 18A2; 42A; calcium Placental protein; Calcium protein; Calvasculin; CAPL; Fibroblast specific protein 1; FSP1; Leukemia multidrug resistance associated protein; Malignant transformation suppression 1; Metastasin; MTS1; Mts1 protein; Murine placental homolog; OTTHUMP00000015467; OTTHUMP00000015468; P9KA; PEL98; Placental calcium binding protein; Placental calcium-binding protein; Protein Mts1; Protein S100 A4; Protein S100-A4; S100 calcium binding protein A4(calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4; S100 calcium-binding protein A4; S100a4; S10A4_HUMAN |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 9E12 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human S100A4 |
| 亞 型 | IgG2a kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the S-100 family. Contains 2 EF-hand domains. SWISS: P26447 Gene ID: 6275 Database links: Entrez Gene: 6275 Human Entrez Gene: 20198 Mouse Omim: 114210 Human SwissProt: P26447 Human SwissProt: P07091 Mouse Unigene: 654444 Human Unigene: 3925 Mouse Unigene: 504 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
