| 產(chǎn)品編號(hào) | bsm-52253R |
| 英文名稱 | NGFR/p75NTR Recombinant Rabbit mAb |
| 中文名稱 | 神經(jīng)生長(zhǎng)因子受體重組兔單抗 |
| 別 名 | TNR16_HUMAN; Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor(NGF receptor 1 Publication); p75 ICD; CD271; TNFRSF16; p75NTR; p75(NTR); Gp80-LNGFR; p75 NGF Receptor; |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | 5D4 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human p75 NTR: 350-400 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis. Function: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Subunit: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1 (By similarity). Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with NRADD. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: N- and O-glycosylated. Phosphorylated on serine residues. Similarity: Contains 1 death domain. Contains 4 TNFR-Cys repeats. SWISS: P08138 Gene ID: 4804 Database links: Entrez Gene: 4804 Human Entrez Gene: 18053 Mouse Omim: 162010 Human SwissProt: P08138 Human SwissProt: Q9Z0W1 Mouse Unigene: 415768 Human Unigene: 681726 Human Unigene: 283893 Mouse Unigene: 10980 Rat 神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology） NGFR神經(jīng)生長(zhǎng)因子受體又稱：CD271存在與細(xì)胞上的與神經(jīng)生長(zhǎng)因子相結(jié)合并相互作用的蛋白質(zhì)因子，屬一型跨膜TNF受體超家族糖蛋白，它可與NGF, BDNF, NT-3, NT-4 結(jié)合，根據(jù)與神經(jīng)生長(zhǎng)因子的結(jié)合力速度及親和力可分為高親和力和低親和力兩種NGFR。 此抗體識(shí)別分子量為47kDa低親和力的NGFR.NGF-R也稱為低親和神經(jīng)生長(zhǎng)因子受體，屬1型跨膜TNF受體超家族。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NGFR/p75NTR Monoclonal Antibody, Unconjugated(bsm-52253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed PC-12 (Rat) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NGFR/p75NTR) monoclonal Antibody, unconjugated (bsm-52253R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green,bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
|
