| 產(chǎn)品編號 | bsm-0954M-HRP |
| 英文名稱 | Mouse Anti-ET-1(2C4)/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的內(nèi)皮素-1單克隆抗體 |
| 別 名 | Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1 |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 心血管 神經(jīng)生物學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 2C4 |
| 交叉反應 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Sheep, Guinea Pig, ) |
| 產(chǎn)品應用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 2.5kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Function: Endothelins are endothelium-derived vasoconstrictor peptides. Subcellular Location: Secreted. Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Similarity: Belongs to the endothelin/sarafotoxin family. Database links: Entrez Gene: 1906 Human Entrez Gene: 13614 Mouse Omim: 131240 Human SwissProt: P05305 Human SwissProt: P22387 Mouse Unigene: 511899 Human Unigene: 713645 Human Unigene: 10918 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 內(nèi)皮素(Endothelin-1,ET)是一種強的血管收縮劑，分為ET-1,ET-2,ET-3 三種,ET-1主要在內(nèi)皮細胞中表達,而ET-2和ET-3則主要在神經(jīng)系統(tǒng)中表達。ET具有廣泛的生物學作用，此抗體是針對內(nèi)皮素的活性片段（CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11）。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
