| 產品編號 | bs-1849R-Gold |
| 英文名稱 | Rabbit Anti-Midkine/Gold Conjugated antibody |
| 中文名稱 | 膠體金標記的中期因子/肝素結合生長因子抗體 |
| 別 名 | Amphiregulin associated protein; ARAP; FLJ27379; MDK; Midgestation and kidney protein; MK 1; MK; MK1; NEGF 2; NEGF2; neurite growth promoting factor 2; Neurite outgrowth promoting protein; MK_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領域 | 腫瘤 免疫學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Fruit Fly, ) |
| 產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 13.4kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Midkine |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產品介紹 |
background: Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines. Function: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity). Subunit: Homodimer. Interacts with ALK. Subcellular Location: Secreted. Similarity: Belongs to the pleiotrophin family. Database links: Entrez Gene: 4192 Human Entrez Gene: 17242 Mouse Omim: 162096 Human SwissProt: P21741 Human SwissProt: P12025 Mouse Unigene: 21528 Cow Unigene: 82045 Human Unigene: 906 Mouse Unigene: 17447 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 中期因子(midkine, MK)具有多種生物學功能: 可促進神經軸突 的生長和各種胚胎神經元的存活,做為一種有絲分裂原,與腫瘤的發(fā)生密切相關，MK還有較強的 促血管生成特性, 經研究證實, MK可促進內皮細胞增殖并 提高血管的密度，在多種腫瘤組織和腫 瘤患者的血清中的水平都明顯升高。MK和多向促激素(p leiotro2 phin, PTN)同屬于一類新的結合肝素的生長因子家 族。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
