| 產(chǎn)品編號 | bs-9456R-Bio |
| 英文名稱 | Rabbit Anti-SIRT5/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的沉默調(diào)節(jié)蛋白5抗體 |
| 別 名 | NAD dependent deacetylase sirtuin 5; NAD-dependent deacetylase sirtuin 5; NAD-dependent deacetylase sirtuin-5; Silent mating type information regulation 2 S.cerevisiae homolog 5; Sir2 like 5; Sir2-like 5; SIR2-like protein 5; SIR2L5; Sirt5; SIRT5_HUMAN; Sirtuin type 5. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 心血管 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, ) |
| 產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from hu SIRT5 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Sirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal RNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT5 (NAD-dependent deacetylase sirtuin-5), also known as SIR2L5, is a 310 amino acid member of the class III sirtuins. Localized to mitochrondria and expressed throughout the body, SIRT5 is an NAD-dependent deacetylase that may link metabolic aging processes in humans. SIRT5 contains one deacetylase-sirtuin-type domain and can be deactivated by suramin, a drug that blocks the binding of various growth factors. Two isoforms of SIRT5 exist due to alternative splicing events. Subcellular Location: Mitochondrion matrix. Mitochondrion intermembrane space. Tissue Specificity: Widely expressed. Database links: Entrez Gene: 23408 Human Entrez Gene: 68346 Mouse Omim: 604483 Human SwissProt: Q9NXA8 Human SwissProt: Q8K2C6 Mouse SwissProt: Q5R6G3 Orangutan Unigene: 567431 Human Unigene: 594133 Human Unigene: 35325 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
