| 產(chǎn)品編號 | bs-20281R-Bio |
| 英文名稱 | Rabbit Anti-HARS/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的組氨酸t(yī)RNA連接酶抗體 |
| 別 名 | EC 6.1.1.21; FLJ20491; HisRS; Jo-1; histidine translase; Histidine tRNA ligase; Histidyl tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HARS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Aminoacyl-tRNA synthetases are a class of enzymes thatcharge tRNAs with their cognate amino acids. The protein encoded bythis gene is a cytoplasmic enzyme which belongs to the class IIfamily of aminoacyl-tRNA synthetases. The enzyme is responsible forthe synthesis of histidyl-transfer RNA, which is essential for theincorporation of histidine into proteins. The gene is located in ahead-to-head orientation with HARSL on chromosome five, where thehomologous genes share a bidirectional promoter. The gene productis a frequent target of autoantibodies in the human autoimmunedisease polymyositis/dermatomyositis. Several transcript variantsencoding different isoforms have been found for this gene. Subcellular Location: Cytoplasmic Tissue Specificity: Brain, heart, liver and kidney. Post-translational modifications: Defects in HARS are a cause of Usher syndrome type 3B(USH3B) [MIM:614504]. USH3B is a syndrome characterized byprogressive vision and hearing loss during early childhood. Somepatients have the so-called 'Charles Bonnet syndrome,' involvingdecreased visual acuity and vivid visual hallucinations. USH is agenetically heterogeneous condition characterized by theassociation of retinitis pigmentosa with sensorineural deafness.Age at onset and differences in auditory and vestibular functiondistinguish Usher syndrome type 1 (USH1), Usher syndrome type 2(USH2) and Usher syndrome type 3 (USH3). USH3 is characterized bypostlingual, progressive hearing loss, variable vestibulardysfunction, and onset of retinitis pigmentosa symptoms, includingnyctalopia, constriction of the visual fields, and loss of centralvisual acuity, usually by the second decade of life. Similarity: Belongs to the class-II aminoacyl-tRNA synthetasefamily. Contains 1 WHEP-TRS domain. Database links: Entrez Gene: 3035 Human Entrez Gene: 15115 Mouse Entrez Gene: 100173931 Orangutan Omim: 142810 Human SwissProt: P12081 Human SwissProt: Q61035 Mouse SwissProt: Q5R4R2 Orangutan Unigene: 528050 Human Unigene: 10528 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
