| 產(chǎn)品編號 | bsm-61179R |
| 英文名稱 | TBP Recombinant Rabbit mAb |
| 中文名稱 | TATA結(jié)合蛋白重組兔單抗 |
| 別 名 | TBP; GTF2D; GTF2D1; HDL4; SCA17; TFIID; TATA binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | 36 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TBP: 235-294 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). SWISS: P20226 Gene ID: 6908 在DNA結(jié)合多蛋白因子TFIID的核心起作用的一般轉(zhuǎn)錄因子。TFIID與TATA盒的結(jié)合是pre-initiation complex（PIC）的初始轉(zhuǎn)錄步驟，在RNA聚合酶II轉(zhuǎn)錄的真核基因激活中起作用。也是一種含有BRF2的轉(zhuǎn)錄因子復合物的成分，調(diào)節(jié)RNA聚合酶III介導的轉(zhuǎn)錄。 |
| 產(chǎn)品圖片 |
Western blot analysis of K562 cell lysate. Using TBP (bsm-61179R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
