| 產(chǎn)品編號 | bs-3874R |
| 英文名稱 | ALOX12 Rabbit pAb |
| 中文名稱 | 12脂氧合酶抗體 |
| 別 名 | 12R-LOX; 12R-lipoxygenase; EC:1.13.11; Epidermis-type lipoxygenase 12; |
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Specific References (3) | bs-3874R has been referenced in 3 publications.
[IF=8.101] Jin Wang. et al. Irisin protects against sepsis-associated encephalopathy by suppressing ferroptosis via activation of the Nrf2/GPX4 signal axis. FREE RADICAL BIO MED. 2022 Jul;187:171 WB ; Mouse.
[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359 WB ; Rat.
[IF=4.932] Zhikun Zhan. et al. Effect of oridonin on oxylipins in the livers of mice with acute liver injury induced by D-galactosamine and lipopolysaccharide. Int Immunopharmacol. 2021 Nov;:108387 WB ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 心血管 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 12 Lipoxygenase: 101-200/663 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017] Function: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:16638750, Subcellular Location: Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF. Tissue Specificity: Expressed in vascular smooth muscle cells. DISEASE: Esophageal cancer (ESCR) [MIM:133239]: A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. {ECO:0000269|PubMed:17460548}. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to esophageal cancer (PubMed:17460548). {ECO:0000269|PubMed:17460548}. Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. {ECO:0000269|PubMed:17151091}. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to colorectal cancer (PubMed:17460548). {ECO:0000269|PubMed:17460548}. Similarity: Belongs to the lipoxygenase family. SWISS: P18054 Gene ID: 239 Database links: Entrez Gene: 239 Human Omim: 152391 Human SwissProt: P18054 Human Unigene: 654431 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Esophagus (Mouse) Lysate at 40 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Lane 5: 293T (Human) Cell Lysate at 30 ug
Lane 6: U937 (Human) Cell Lysate at 30 ug
Lane 7: K562 (Human) Cell Lysate at 30 ug
Primary: Anti- ALOX12 (bs-3874R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
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