| 產(chǎn)品編號(hào) | bsm-2271M |
| 英文名稱 | HCMV(1F11) Mouse mAb |
| 中文名稱 | 人巨細(xì)胞病毒單克隆抗體 |
| 別 名 | CMV; Cytomegalovirus; CMV glycoprotein B; CMV glycoprotein GP55; Cytomegalovirus glycoprotein GP55; gB; Glycoprotein B; Glycoprotein GP55; HCMV; HCMV glycoprotein B; HCMV glycoprotein GP55; HHV 5; HHV 5 glycoprotein B; HHV 5 glycoprotein GP55; HHV5 glycoprotein B; HHV5 glycoprotein GP55; Human herpesvirus 5 glycoprotein B; Human herpesvirus 5 glycoprotein GP55; UL55. |
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Specific References (1) | bsm-2271M has been referenced in 1 publications.
[IF=3.939] Mohammed Amjed Alsaegh . et al. Human cytomegalovirus is present in the odontogenic epithelium of ameloblastoma. J Oral Microbiol. 2021;13(1):1874699 IHC ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 1F11 |
| 交叉反應(yīng) | (predicted: HCMV) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant HCMV protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens. Function: Counteracts the host antiviral immune response by preventing IRF3 to enter the nucleus once activated and phosphorylated. Participates also in the transactivation of viral major imediate-early genes by recruiting host IFI16 to their promoters. Subunit: Interacts with host NCL/nucleolin. Interacts with host IFI16. Subcellular Location: Virion tegument (Potential). Host nucleus. Host cytoplasm. Note=As part of the incoming virion, pp65 is targeted to the nucleus immediately after infection. The newly synthesized pp65 is observed in the nucleus until some time after 48 hours postinfection. Thereafter, pp65 is probably exported and accumulates in the cytoplasm. Also found in dense bodies. Post-translational modifications: Phosphorylation may play a role in the localization of the protein. Similarity: Belongs to the herpesviridae pp65 family. Database links: Entrez Gene: 3077579 Human herpesvirus 5 CMV又稱為涎病毒，屬于皰疹病毒亞科，是人類皰疹病毒組中最大的一種病毒。由分子量約為150X106的線狀雙股DNA所組成。其形最大由162個(gè)殼粒（capsomer），正20面體構(gòu)成。有典型的皰疹病毒結(jié)構(gòu)。形態(tài)與單純皰疹病毒及水痘-帶狀皰疹病毒非常相似，不易區(qū)別。 CMV只能在人纖維母細(xì)胞的組織培養(yǎng)中增殖，而不能在其他動(dòng)物細(xì)胞中生長，增殖非常緩慢，其復(fù)制周期為36-48小時(shí)，比單純皰疹病毒復(fù)制周期8小時(shí)要長得多。初次分離需一個(gè)多月才能出現(xiàn)特殊的細(xì)胞；細(xì)胞變園，膨脹，細(xì)胞及核巨大化，核周圍出現(xiàn)一輪“暈”的大型嗜酸性包涵體。在活體中的靶細(xì)胞主要是上皮細(xì)胞。人類CMV各株之間有廣泛交叉反應(yīng)。 CMV在20%乙醚中最多存活2小時(shí)。pH<5時(shí)，或置于56℃30分鐘，或紫外線照射5分鐘可被充分滅活。CMV的感染性對(duì)凍融或存于-20℃或-50℃均不穩(wěn)定，10%的家用漂白粉可使其感染性明顯降低。 CMV感染的特征時(shí)出現(xiàn)有典型的胞漿及核內(nèi)包涵體的巨大細(xì)胞，故又名巨細(xì)胞病毒。它在人體組織中可形成肥大的細(xì)胞，引起巨細(xì)胞包涵體病。 巨細(xì)胞病毒感染可導(dǎo)致致畸 人巨細(xì)胞病毒是一種雙鏈DNA病毒，是最大的動(dòng)物病毒之一。人是人巨細(xì)胞病毒唯一可感染的對(duì)象。同其他病毒相比，由于人巨細(xì)胞病毒的毒力較弱，侵入機(jī)體后一般不會(huì)使器官和組織受到嚴(yán)重?fù)p傷，但是該病毒基因整合在受精卵細(xì)胞的相關(guān)基因后，則可阻止或影響后者的復(fù)制和表達(dá)，最后導(dǎo)致不可逆的形態(tài)和結(jié)構(gòu)的異常。 巨細(xì)胞病毒主要在早孕3個(gè)月內(nèi)感染致胎兒畸形，腦是巨細(xì)胞病毒最易侵襲的器官，胎兒腦組織中的神經(jīng)元細(xì)胞、神經(jīng)膠質(zhì)細(xì)胞均可受侵犯。顱內(nèi)鈣鹽沉著、腦軟化、腦積水是人巨細(xì)胞病毒感染最常見的臨床表現(xiàn)，其后遺癥也以神經(jīng)系統(tǒng)最為嚴(yán)重。最常見的是智力低下，其他如粗大運(yùn)動(dòng)異常，精神運(yùn)動(dòng)異常，感覺神經(jīng)性耳聾，脈絡(luò)膜視網(wǎng)膜炎等。雖然在聽力和神經(jīng)方面明顯存在著高風(fēng)險(xiǎn)，甚至可能有后遺癥，只有5%的被感染嬰兒會(huì)在新生兒期就表現(xiàn)巨細(xì)胞病毒感染征象。而90%的先天性感染的胎兒出生時(shí)無任何臨床癥狀。因此，加強(qiáng)監(jiān)測，盡早診斷是預(yù)防巨細(xì)胞病毒感染致畸的重要措施。懷孕時(shí)初次感染巨細(xì)胞病毒的孕婦，其胎兒的感染率是30%，而被感染的孕婦在分娩時(shí)有40%可傳染給胎兒。妊娠期，巨細(xì)胞病毒感染至今尚無特殊療法，所以關(guān)鍵在預(yù)防。對(duì)早孕婦女，不僅可監(jiān)測其抗體，還可用特殊方法監(jiān)測其抗原，如果發(fā)現(xiàn)孕婦體內(nèi)有巨細(xì)胞病毒活躍復(fù)制，應(yīng)采取果斷措施，終止妊娠，以避免畸形兒出生。此外，要提高人們的文化素質(zhì)，講究衛(wèi)生，杜絕病毒感染的各種機(jī)會(huì)，并注意保持和增強(qiáng)人體免疫功能，這樣就有可能防止或減少育齡婦女巨細(xì)胞病毒感染。 巨細(xì)胞包涵體病對(duì)小兒腦組織廣泛損傷-智力障礙 巨細(xì)胞包涵體病是由于巨細(xì)胞病毒感染后，引起的全身多個(gè)器官損害并出現(xiàn)臨床癥狀的疾病。若在出生時(shí)就有臨床癥狀，則為宮內(nèi)感染(先天感染)，出生后數(shù)天或數(shù)周發(fā)病多為出生后感染。巨細(xì)胞病毒感染很廣泛，是先天病毒感染的重要病原之一。巨細(xì)胞病毒感染可引起胎兒腦組織廣泛損傷，是小兒智力低下的最主要原因。 巨細(xì)胞病毒感染較廣泛，大多數(shù)人一生中不同時(shí)期均可獲得感染。居住擁擠、經(jīng)濟(jì)條件差、衛(wèi)生條件差的人易感染。女性較同齡男性感染率高。晚期孕婦宮頸排出巨細(xì)胞病毒者達(dá)28% ，是先天性巨細(xì)胞病毒感染的重要原因。母乳排毒者約13%～27%，且排毒時(shí)間長，易引起后天感染。 巨細(xì)胞病毒感染后，臨床表現(xiàn)有多種類型。如為先天感染，出生后即有低體重，呼吸不規(guī)則，黃疸重，肝臟、脾臟腫大，抽搐，視力受損，肌肉癱瘓，智力低下等。如為后天感染，一般癥狀較輕，多表現(xiàn)為肝炎癥狀。 因此，育齡婦女懷孕前應(yīng)做體檢，如果發(fā)現(xiàn)有巨細(xì)胞病毒感染應(yīng)抗病毒治療，使血中巨細(xì)胞病毒抗體轉(zhuǎn)陰，子宮頸分泌物檢查巨細(xì)胞病毒為陰性方可受孕。 |
