| 產(chǎn)品編號 | bsm-61012R |
| 英文名稱 | Glucose 6 phosphate isomerase Recombinant Rabbit mAb |
| 中文名稱 | 糖磷脂酰肌醇重組兔單抗 |
| 別 名 | AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PHI; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 3A14 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human GPI: 1-64/558 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq, Jul 2008]. Function: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Subunit: Homodimer in the catalytically active form, monomer in the secreted form. Subcellular Location: Cytoplasm. Secreted. Post-translational modifications: Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated. DISEASE: Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Similarity: Belongs to the GPI family. Database links: Entrez Gene: 2821 Human Entrez Gene: 14751 Mouse Omim: 172400 Human SwissProt: P06744 Human SwissProt: P06745 Mouse Unigene: 466471 Human Unigene: 589 Mouse Unigene: 84435 Rat 糖基化磷脂酰肌醇(GPI)錨定蛋白是一種新型細(xì)胞表面蛋白,又稱:糖基磷脂酰肌醇錨定蛋白質(zhì),它只通過GPI結(jié)構(gòu)錨定于細(xì)胞膜表面而不跨越其磷脂膜雙層結(jié)構(gòu);當(dāng)它與細(xì)胞共同孵育時(shí),可自動(dòng)整合至細(xì)胞膜表面。GPI錨定蛋白轉(zhuǎn)化法利用GPI錨定信號將目的蛋白直接整合至靶細(xì)胞表面,超越了目的蛋白由靶細(xì)胞自身合成的機(jī)制,具有對靶細(xì)胞選擇性不高,轉(zhuǎn)化過程迅速等優(yōu)點(diǎn). |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Glucose 6 phosphate isomerase monoclonal antibody, unconjugated (bsm-61012R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated(bsm-61012R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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