| 產(chǎn)品編號(hào) | bsm-63277R |
| 英文名稱 | phospho-eIF4EBP1（T70） Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化eIF4E結(jié)合蛋白重組兔單抗 |
| 別 名 | 4EBP1; eIF4E binding protein 1; Eif4ebp1; PHASI. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 6G2 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測(cè)分子量 | 17 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human 4E BP1 around the phosphorylation site of T70: TK-pT-PPR |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. SWISS: Q13541 Gene ID: 1978 4E-BP1是一種分子量為10-12kDa 的酸性蛋白，與eIF4G 競(jìng)爭(zhēng)結(jié)合。4E-BPs 對(duì) eIF4E 的結(jié)合是可逆的，并依賴于 4E-BP 的磷酸化狀態(tài)。非磷酸化的4E-BP1 能與 eiF4E 牢固結(jié)合，而磷酸化的4E-BP1則不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通過對(duì)35、45、69位的蘇氨酸殘基或64位的絲氨酸殘基進(jìn)行磷酸化而使4E-BP1喪失對(duì)eIF4E結(jié)合能力的激酶。然而，并非所有的磷酸化事件都能 |
